e-ISSN 1694-2078
p-ISSN 1694-2086

Arch Med Biomed Res. 2014;1:156-162. doi:10.4314/ambr.v1i4.5

Naushad A Toolsee1, Okezie I Aruoma2, Philippe Rondeau3, Emmanuel Bourdon3, Theeshan Bahorun1

Author Affiliations

1ANDI Centre of Excellence for Biomedical and Biomaterials Research and Department of Biosciences University of Mauritius, Réduit, Republic of Mauritius
2School of Pharmacy, American University of Health Sciences, Signal Hill, CA, USA
3Groupe d'Etude sur l'Inflammation Chronique et l'Obésité, Université de La Réunion, Plate-forme CYROI, Saint Denis, La Réunion, France

correspondence to
Theeshan Bahorun; tbahorun@uom.ac.mu
Okezie I Aruoma; oaruoma@auhs.edu

Received: October 23, 2014
Revised: November 1, 2014
Accepted: November 3, 2014


The consumption of green tea is epidemiologically associated with a reduction in diabetic nephropathy; however its prophylactic effect remains unclear in an oxidative stress-associated diabetic milieu. The energy metabolism of HEK-293 cell, pretreated with variable concentrations of green tea, was evaluated under different hydrogen peroxide (H2O2) concentrations using the MTT assay. Green tea modulated the energy metabolism in renal cell line under different hydrogen peroxide challenge. In the absence of hydrogen peroxide, green tea at concentrations of 0.25 mg/mL and 0.50 mg/mL significantly increased the energy metabolism of HEK-293 cell by 81.5 % (P < 0.01) and 56.1 % (P < 0.05), respectively. Green tea at a concentration of 3 mg/mL significantly reduced (P < 0.05) the energy metabolism in HEK-293 cell by 36.6 % and 20.7 % when incubated in the presence of 200 µM and 500 µM H2O2, respectively. At high concentration, H2O2 and green tea have shown the ability in reducing the energy metabolism of HEK-293 cell. Conclusively, moderate consumption of green tea could form part of a healthy lifestyle that might ameliorate features of metabolic syndrome and subsequent risks for diabetic nephropathy, an outcome that can be determined by further clinical studies.

KEY WORDS: Diabetes; Green tea and wellness; Antioxidant; Nephropathy; Metabolic syndrome


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